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ABS-933 Principles of Molecular Biology
Campus ASAB
Programs PG
Session Fall Semester 2016
Course Title Principles of Molecular Biology
Course Code ABS-933
Credit Hours 3-0
Course Objectives This course covers recent advances in the field of Molecular biology including the structure, function, and interaction of various proteins and nucleic acids. Furthermore some of research articles in the scientific literature that provide the discoveries and methods critical to modern molecular and cell biology will be reviewed during the course. Principles and practice of classical and molecular methods as applied to eukaryotic organisms; isolation and characterization of mutations; gene mapping; suppressor analysis; and, other techniques for studying chromosome structure, gene expression and developmental processes will also be covered in this course.
Detail Content
  • Molecular structure and organization of Genes, Genome and Chromosomes
  • Gene Trancommention
  • Regulation of Trancommention Initiation, role of GTF and TSFs
  • DNA Replication, Recombination (site specific and non-homologous)
  • RNA Processing, Nuclear Transport, and Post-Trancommentional Control
  • Control of Translation
  • mRNA degradation and interference
  • Molecular pathology
    • Molecular pathology: from gene to disease
    • Molecular pathology: from disease to gene
    • Molecular pathology of chromosomal disorders
  • Studying human gene structure, expression and function using cultured cells and cell extracts
    • Studying gene expression using cultured cells or cell extracts
    • Identifying regulatory sequences through the use of reporter genes and DNA-protein interactions
    • Investigating gene function by identifying interactions between a protein and other macromolecules
  • Genetic manipulation of animals
    • The creation and applications of transgenic animals
    • Use of mouse embryonic stem cells in gene targeting and gene trapping
    • Manipulating animals by somatic cell nuclear transfer.
  • Gene Promoters
  • Gene Enhancers
  • Structure, function and regulation of chromatin network
  • Linker proteins
  • Histone proteins and the role acetylation and deacetylation in trancommention.
  • Models for Molecular Biology research and the rationale of using these models
    • Arabidospsis thaliana
    • C. elegans
    • Zebra fish
    • Drosophila
    • Small rodents (rats/mice)
    • Cell models like Chinese hamster ovary cells (CHO), HEK 293 and HeLa cells.
Text/Ref Books
  1. Molecular Cell Biology by Lodish, Molecular Cell Biology
  2. Human Molecular Genetics 2 by Tom Strachan & Andrew P. Read
  3. RNA and the Regulation of Gene Expression: A Hidden Layer of Complexity by Kevin V. Morris The Scripps Research Institute, La Jolla, USA
  4. Genomic Technologies: Present and Future by David J. Galas Keck Graduate Institute, Claremont, CA
  5. Molecular Biology of the Gene by Watson, J. D., T. A. Baker, S. P. Bell, A. Gann, M. Levine, and R. Losick, Eds., (2003). (5th edition) New York, Benjamin Cummings
  6. Cell and Molecular Biology by Gerald Karp.1996 John Willey and Sons, Inc. London.
Time Schedule Fall Semester 2014
Faculty/Resource Person Dr. Peter John
PhD (QAU Islamabad)

Discipline: Molecular Biology