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HCB-910 Cell Cycle Regulation
Campus ASAB
Programs PG
Session Fall Semester 2016
Course Title Cell Cycle Regulation
Course Code HCB-910
Credit Hours 3-0
Course Objectives This course is intended to provide foundational information biology of cancer cell, cell division and cell cycle checkpoint. The main theme centers around cell cycle checkpoints with specific emphasis on the biochemistry and genetics of DNA damage signaling pathways, DNA damage checkpoints, mitotic checkpoints and their relevance to human cancer.
Detail Content
  • Introduction to cell
  • Cells and genome
  • Basic Genetic mechanisms
    • DNA and chromosome
    • Chromosomal DNA and its packaging into chromatin
    • Global structure of chromosome
  • Overview of cell cycle and role played by each kind of CDK and respective cyclins in each phase namely (G1, S, G2 and M)
  • Components of cell cycle control system
    • Cyclins
    • Cyclin-dependent Kinases (CDKs)
    • Cyclin-Cdk regulation
    • CDK activating kinases (CAKs)
    • CDK inhibiting kinases (CKIs)
    • Regulation of cyclins through proteolysis (Ubiquitination, APC/C and SCF E3 ligases)
  • Checkpoint control of the cell cycle
  • Cell cycle control – the role of the tumor suppressor pRb in the G1/S transition
    • p53 regulation
    • G1 to G0 and G0 to G1 re-entry; the decision making involved
  • Biology of cancer cell/ Hallmarks of cancer
  • The DNA damage checkpoint depending on cell cycle stage (DNA damage sensor proteins ATM, ATR and DNAPK)
  • DNA Breaks and the Cell Cycle:Checkpoints and Cell Cycle Arrest
  • S-phase events:
    • o DNA replication
    • Pre-replication complex and “Licensing.”
    • Origin of replication in Fission yeast and Higher Metazoans
    • End replication problem
    • Restoration of chromatin structure
    • Deposition of Cohesin
    • Replication of Centrosomes
  • G2 phase events:
    • Changes in microtubule dynamic properties.
    • Chromosome condensation
    • Building of kinetochore
    • Triggering activity of Cyclin B/CDK1 and MPF to stimulate G2-M transition.
    • Power play between CAK/Cdc25/Myt1/wee1 to regulate Cyclin B/Cdk1.
    • Role of Condensin protein
    • G2-M DNA damage checkpoint
  • Main events of M-phase
    • Inhibition of protein synthesis due to phosphorylation of E2Fa.
    • Activation of MLC phosphatase by Cyclin B/Cdk1 to inactivate Myosin II.
    • Decline in major cell membrane functions.
    • Golgi apparatus vesiculation.
    • Separation of centrosomes.
      • Accumulation of kinetochore proteins on centromeric DNA.
    • Microtubule dynamics at M-phase.
  • 'To die or not to die' – the decision between repair and apoptosis
  • Checkpoint-related syndromes
    • Nijmegen breakage syndrome
    • Bloom's syndrome
    • Familial cancer syndromes
  • Programmed cell death (Apoptosis)
    • Intrinsic Pathway
    • Extrinsic Pathway
Text/Ref Books
  1. Molecular Biology of the cell by Alberts et al. 2005
  2. Cell Biology by Thomas D. Pollard and William C. Earnshaw (3rd edition).
  3. The Biology of Cancer by Robert A. Weinberg
  4. Cell Cycle and Growth Control: Biomolecular Regulation and Cancer, Gary S. Stein, Arthur B. Pardee.
Time Schedule Fall Semester 2014
Faculty/Resource Person Dr. Nasir Jalal
PhD (Colorado State University)

Discipline: Cell and Molecular Biology