NUST - PG Courses

IBT-820 Industrial Biotechnology & Microbiology

Campus	ASAB
Programs	PG
Session	Fall Semester 2016
Course Title	Industrial Biotechnology & Microbiology
Course Code	IBT-820
Credit Hours	3
Pre-Requisutes	None
Course Objectives	To provide an overview and discuss details of significant processes and products in biotechnological industries and commercial biology based activities. To impart the knowledge on Historical overview of Biotechnology, production of some commercially important modern Bioproducts, Industrial Enzymes, Products of plant and animal cell cultures. Production of recombinant proteins.
Detail Content	Introduction to industrial bioprocess A historical overview of industrial fermentation process – traditional and modern biotechnology. A brief survey of organisms, processes, products relating to modern biotechnology. Process flow sheeting – block diagrams, pictorial representation. Production of primary metabolites A brief outline of processes for the production of some commercially important organic acids (e.g. citric acid, lactic acid, acetic acid etc.,); amino acids (glutamic acid, phenyalanine, aspartic acid etc.,) and alcohols (ethanol, butanol etc.,) Production of secondary metabolites Study of production processes for various classes of secondary metabolites antibiotics beta-lactams (penicillin, cephalosporin etc.), aminoglycosides (streptomycin etc.,) macrolides (erythromycin), vitamins and steroids. Production of enzymes and other bioproducts Production of industrial enzymes such as proteases, amylases, lipases, cellulases etc., Production of biopesticides, biofertilisers, biopreservatives (Nisin), cheese, biopolymers (xanthan gum, PHB etc.,), single cell protein. Production modern biotechnology products Production of recombinant proteins having therapeutic and diagnostic applications, production of vaccines. Production of monoclonal antibodies. Products of plant and animal cell culture Microbiolog Microbial groups (bacteria, protozoa, microscopic algae, fungi, cyanobacteria, virus). Applied fields of microbiology (medical, aquatic, food, agricultural, geochemical, acromicrobilogy, industrial, exomicrobilogy). Cell types – structures of some prokaryotes and eukaryotes. Structures and functions of cell organelles Differences between prokaryotes and eukaryotes. Cell differention, specialiazation and function, Taxanomic groups, Classification Methods e.g., for (Bacteria). Microbial Control, Preservation, and Maintenance Control & Preservation methods. Maintenance -Features to be considered (e.g cost, viability, purity, Genetic, etc) -Methods (subcluture, drying freeze drying, freezing , etc, Microbial metabolism Growth and growth measurement- Nutritional groups with respect to carbon and energy requirements (photoautotrophs, photoheterotrophs, chemoautotrophs,chemoheterotrophs). Type of microbial culture, setting up and maintaining them. Selection of organisms and optimization of yield of product Microorganism Growth in Controlled Environments, Medium Development Growth of Microorganisms in an Industrial Setting insulin - downstream processing after E. coli fermentation Alcoholic beverage industry-Beer brewing and Wine-making History and current practices, “Secondary” fermentations and their uses in wine making Genetic engineering of yeast strains to address key industry problems Industrial ethanol production, Methods, sources of feed stocks used Current and potential microorganisms used Biochemical and microbiological issues in maximizing ethanol production Markets for ethanol and an analysis of economic and environmental issues Yeast production for the food industry – the transition from a beer-making by-product Fermented food industry -Food and beverage production (dairy products, bread making, mushroom growing, etc). Antibiotic production, Microbial enzyme industry
Text/Ref Books	Casida Jr, L.E., “Industrial Microbiology”, New Age International (P) Ltd. Presscott, Dunn, “Industrial Microbiology”, Agrobios (India). Wulf Cruger and Anneliese Crueger, “Biotechnology A Textbook of Industrial Microbiology”, Panima Publishing Corporation. Murrey Moo & Young, “Comprehensive Biotechnology”, Pergamon. Industrial Microbiology An Introduction by Michael J. Waites, Neil L. Morgan, John S. Rockey and Gary Higton. Blackwell Sciences. 2001 Modern Industrial Microbiology and Biotechnology by NdukaOkafor 2007 Manual of Industrial Microbiology & Biotechnology by Baltz et al. 2010
Time Schedule
Faculty/Resource Person	Assistant Professor – Dr. Husnain Ahmed (PhD)