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ABS-933 Principles of Molecular Biology
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Campus
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ASAB
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Programs
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PG
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Session
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Fall Semester 2016
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Course Title
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Principles of Molecular Biology
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Course Code
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ABS-933
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Credit Hours
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3
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Pre-Requisutes
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None
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Course Objectives
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- This course covers recent advances in the field of Molecular biology including the structure, function, and interaction of various proteins and nucleic acids.
- Furthermore some of research articles in the scientific literature that provide the discoveries and methods critical to modern molecular and cell biology will be reviewed during the course.
- Principles and practice of classical and molecular methods as applied to eukaryotic organisms; isolation and characterization of mutations; gene mapping; suppressor analysis; and, other techniques for studying chromosome structure, gene expression and developmental processes will also be covered in this course.
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Detail Content
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- Molecular structure and organization of Genes, Genome and Chromosomes
- Gene Trancommention, Regulation of Trancommention Initiation, role of GTF and TSFs
- DNA Replication, Recombination (site specific and non-homologous)
- RNA Processing, Nuclear Transport, and Post-Trancommentional Control
- Control of Translation, mRNA degradation and interference
- Molecular pathology
- Molecular pathology from gene to disease
- Molecular pathology from disease to gene
- Molecular pathology of chromosomal disorders
- Studying human gene structure, expression and function using cultured cells and cell extracts
- Studying gene expression using cultured cells or cell extracts
- Identifying regulatory sequences through the use of reporter genes and DNA-protein interactions
- Investigating gene function by identifying interactions between a protein and other macromolecules
- Genetic manipulation of animals
- The creation and applications of transgenic animals
- Use of mouse embryonic stem cells in gene targeting and gene trapping
- Manipulating animals by somatic cell nuclear transfer.
- Gene Promoters
- Gene Enhancers
- Structure, function and regulation of chromatin network
- Linker proteins
- Histone proteins and the role acetylation and deacetylation in trancommention.
- Models for Molecular Biology research and the rationale of using these models
- Arabidospsis thaliana
- C. elegans
- Zebra fish
- Drosophila
- Small rodents (rats/mice)
- Cell models like Chinese hamster ovary cells (CHO), HEK 293 and HeLa cells.
Course Outcomes
- To allow students to think beyond the ordinary and learn advanced scientific techniques of molecular biology.
- Acquaint students with the most recent and evolving strategies of molecular biological research.
- Compare advanced techniques to old ones and offset the barriers set by the old.
- Improve the quality of indigenous research to bring it at par with the research of most advanced institutions/organizations.
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Text/Ref Books
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- Molecular Cell Biology by Lodish, Molecular Cell Biology
- Human Molecular Genetics 2 by Tom Strachan & Andrew P. Read
- RNA and the Regulation of Gene Expression A Hidden Layer of Complexity by Kevin V. Morris The Scripps Research Institute, La Jolla, USA
- Genomic Technologies Present and Future by David J. Galas Keck Graduate Institute, Claremont, CA
- Molecular Biology of the Gene by Watson, J. D., T. A. Baker, S. P. Bell, A. Gann, M. Levine, and R. Losick, Eds., (2003). (5th edition) New York, Benjamin Cummings
- Cell and Molecular Biology by Gerald Karp.1996 John Willey and Sons, Inc. London.
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Time Schedule
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Faculty/Resource Person
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Assistant Professor – Dr Hajra Sadia (PhD)
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