National University of Sciences and Technology
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PBT-800 Plant Biotechnology and Genetic Engineering
Campus ASAB
Programs PG
Session Fall Semester 2016
Course Title Plant Biotechnology and Genetic Engineering
Course Code PBT-800
Credit Hours 3
Pre-Requisutes None
Course Objectives
  • The course is designed to give an overview of the Impact of biotechnology with special emphases on molecular aspects of biotechnology and genetics.
  • To become familiar with the recombinant techniques used to manipulate and designed vectors and constructs.
  • Also, it will help to know about basic and required traits in transgenic plant and how to introduce the specific traits to a transgenic plant. A theme is to know about the future of plant biotechnology.
Detail Content
  • Plant Agriculture The Impact of Biotechnology;
  • Biotechnology Crop Planting
    • Why Farmers Use Biotech Crops
    • How the Adoption of Plant Biotechnology has Impacted Environment?
  • Molecular Genetics of Gene expression
    • The gene
    • DNA Packaging into Eukaryotic Chromosomes
    • Trancommention
    • Translation and Protein Posttranslational modifications.
  • Recombinant DNA
    • Vector Design and Construction
    • DNA modification
    • DNA Vector
    • Vector Design
    • Safety Features in Vector Design
  • Genes and Trait of Interest for Transgenic Plants
    • Identification of Gene of Interest via Genomic Studies
    • Traits for Improved Crop Production and Traits for Improved Products and Food Quality.
  • Transgenic Plant Production
    • Basic Concepts of successful Gene Transfer to Plant Cells
    • Agrobacterium,
    • Particle Bombardment.
  • The Future of Plant Biotechnology
    • Site Specific Recombination Systems
    • Zinc-Finger Nucleases
    • The Future of Foods, Fuel and Pharmaceuticals.
Text/Ref Books
  • Plant Biotechnology and Genetics; Principles, Techniques and Applications. Edited by C. Neal Stewart Jr.
  • Basic Biotechnology by Colin Ratledge, Bjorn Kristiansen. Cambridge University Press.
  • Introduction to Biotechnology by William J. Thieman, Michael A. Palladino, Benjamin Cummings.
  • Biotechnology an Introduction by Susan R. Barnum. Brooks Cole.
Time Schedule
Faculty/Resource Person Assistant Professor – Dr Muhammad Tahir (PhD)